ReproCELL KOKEN AteloCell®

AteloCell 部分產品可與幹細胞一起在體內培養。物理性質和 collagen 幾乎相同,此外抗原性低,幾乎沒有免疫反應。多種形態和密度可選擇,適合各種研究。將幹細胞培養在此 3D 結構,再放到老鼠的腎臟損傷處,可正常分化長。腎臟細胞修補損傷,若是直接將幹細胞植入腎臟損傷處,易形成腫瘤。適合各式研究所需。

KOKEN Reprocell
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  • 部分產品可與幹細胞一起在體內培養。
  • 使用修飾過的膠原蛋白作為材料,物理性質和 collagen 幾乎相同,此外抗原性低,幾乎沒有免疫反應。
  • 多種形態和密度可選擇,還有片狀和液態,適合各種研究。
  • 實驗測試,將幹細胞培養在此 3D 結構,再放到老鼠的腎臟損傷處,可正常分化長。
  • 腎臟細胞修補損傷,若是直接將幹細胞植入腎臟損傷處,易形成腫瘤。適合各式研究所需。

Atelocollagen由蛋白酶分解collagen (膠原蛋白)而來,是一種醣蛋白。物理性質和collagen幾乎相同,此外抗原性低,幾乎沒有免疫反應。因為大部分的collagen分子都是由G-X-Y的氨基酸序列組成,物種間差異很小,而抗原被辨識的位置是在兩端的端肽,其中不包括G-X-Y序列。當端肽被去除之後,atelocolagen的抗原性比collagen更低。

海棉狀 Sponge

Atelocollagen, Honeycomb sponge

Atelocollagen coated β-TCP scaffold

Atelocollagen sponge, MIGHTY

Collagen sponge for 35mm culture dish

膜狀 Membrane

Atelocollagen, Permeable membrane for 50mm culture dish, 6-well/24-well culture plate

微球體 Microspheres

Collagen microspheres

試劑 Solutions


Atelocollagen sponge,membrane,powder 由牛的真皮層萃取的膠原蛋白純化而成


KKN-CSH-10Atelocollagen Honeycomb sponge (sterile)100 mg/btl
KKN-CSH-96Atelocollagen Honeycomb Disc 96 (sterile)25 pcs/btl
KKN-ACB-05SAtelocollagen coated β-TCP scaffold (sterile) Disc size : Φ3×1 mm10 pcs/btl
KKN-CSM-25Atelocollagen sponge, MGHTY 25 pcs (sterile)25 mg/btl
KKN-CSM-50Atelocollagen sponge, MGHTY 50 pcs (sterile)50 pcs/btl
KKN-CS-35collagen sponge for 35mm culture dish (sterile)​5 pc/bag​
KKN-MEN-01Atelocollagen permeable membrane for 50 mm culture dish (sterile)5 pcs/box
KKN-CM-6Atelocollagen membrane for 6-well culture plate (sterile)24 pcs/box
KKN-CM-24Atelocollagen membrane for 24-well culture plate (sterile)24 pcs/box
KKN-MIC-00Collagen microspheres (sterile) Size: 100-400 μm, About 3,000,000 particles, Total surface area: about 3,800 cm2/15 ml15 mL/btl

試劑 Solutions

KKN-IPC-30Atelocollagen, Bovine dermis, 3mg/mL (sterile)50 mL/btl
KKN-IPC-50Atelocollagen, Bovine dermis, 5mg/mL (sterile)50 mL/btl
KKN-IAC-30Native collagen, Bovine dermis, 3mg/mL (sterile)50 mL/btl
KKN-IAC-50Native collagen, Bovine dermis, 5mg/mL (sterile)50 mL/btl
KKN-MEN-02Atelocollagen, Eagle’s MEM (sterile)20 mL/btl
KKN-DME-02Atelocollagen, DMEM Low Glucose (sterile)20 mL/btl
KKN-DME-02HAtelocollagen, DMEM High Glucose (sterile)20 mL/btl
KKN-RPM-02Atelocollagen RPMI 1640 (sterile)20 mL/btl
KKN-CL-22Type II collagen (sterile) from bovine cartilage10 mL/btl


KKN-CLS-01Atelocollagen sponge1 pc/bag
KKN-CLF-01Atelocollagen membrane1 sheet/bag
KKN-CLP-01Atelocollagen powder500 mg/btl


Atelocollagen Honeycomb sponge

  1. Zaminy A, et al. Mesenchymal stem cells as an alternative for Schwann cells in rat spinal ord injury. (2013) Iran Biomed J. 17(3):113-122.
  2. Buser Z, et al. Inflammatory response of intervertebral disc cells is reduced by fibrin sealant scaffold in vitro. (2012) J Tissue Eng Regen Med. [Epub ahead of print]
  3. Guo Z, et al. Three-Dimensional Geometry of Honeycomb Collagen Promotes Higher Beating Rate of Myocardial Cells in Culture. (2012) Artif Organs. 36(9):816-819.
  4. Ishii I, et al. Correlation between antizyme 1 and differentiation of vascular smooth muscle cells cultured in honeycomb-like type-I collagen matrix. (2012) Amino Acids. 42(2-3):565-575.
  5. Suzuki T, et al. Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. (2009) Microvasc Res. 77(2):143-149.
  6. (Japanese only) 八巻真理子 I型コラーゲンの人工細胞外マトリックスはマウス胚性幹細胞の腫瘍化能を抑制する。(2009) 日本再生医療学会雑誌 8(1):109-114.
  7. Fukushima K, et al. The axonal regeneration across a honeycomb collagen sponge applied to the transected spinal cord. (2008) J Med Dent Sci. 55(1):71-79.
  8. Imamura T, et al. Three-dimensional culture systems for differentiation of mice embryonic stem cells into hepatocyte-like cells. (2008) J Organ Dysfunct. 4(1):57-64.
  9. Kakudo N, et al. Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. (2008) J Biomed Mater Res A. 84(1):191-197.
  10. Saeki K, et al. Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells. (2008) J Cell Physiol. 217(1):261-280.
  11. Takeuchi R, et al. Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study. (2008) Arthritis Res Ther. 10(4):R77.
  12. Hidetsugu T, et al. Mechanism of bone induction by KUSA/A1 cells using atelocollagen honeycomb scaffold. (2007) J Biomed Sci. 14(2):255-263.
  13. George J, et al. Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds. (2006) Biotechnol Bioeng. 95(3):404-411.
  14. Imamura T, et al. Embryonic stem cell-derived embryoid bodies in three-dimensional culture system form hepatocyte-like cells in vitro and in vivo. (2004) Tissue Eng. 10(11-12):1716-1724.
  15. Itoh H, et al. A honeycomb collagen carrier for cell culture as a tissue engineering scaffold. (2001) Artif Organs. 25(3):213-217.
  16. Moriyama T, et al. Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications. (2001) Tissue Eng. 7(4):415-427.

Atelocollagen sponge, MGHTY

  1. Shiozaki Y, et al. Enhanced in vivoosteogenesis by nanocarrier-fused bone morphogenetic protein-4. (2013) Int J Nanomedicine. 8:1349-1360.
  2. Akamine Y, et al. Prolonged matrix metalloproteinase-3 high expression after cycliccompressive load on human synovial cells in three-dimensional cultured tissue. (2012) Int J Oral Maxillofac Surg. 41(7):874-881.
  3. Muroi Y, et al. Effects of compressive loading on human synovium-derived cells. (2007) J Dent Res. 86(8):786-791.

Atelocollagen membrane for 6-well culture plate (sterile)

Atelocollagen membrane for 24-well culture plate (sterile)

  1. Lu H, et al. Comparison of decellularization techniques for preparation of extracellular matrix scaffolds derived from three-dimensional cell culture. (2012) J Biomed Mater Res A. 100(9):2507-2516.
  2. Kobayashi Y, et al. Synthesis of functional artificial lymphoid tissues. (2011) Discov Med. 12(65):351-362.
  3. Kitajima T, et al. A fusion protein of hepatocyte growth factor for immobilization to collagen. (2007) Biomaterials. 28(11):1989-1997.
  4. Okamoto N, et al. Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient mice. (2007) J Clin Invest. 117(4):997-1007.
  5. Ueno A, et al. Constitutive expression of thrombospondin 1 in MC3T3-E1 osteoblastic cells inhibits mineralization. (2006) J Cell Physiol. 209(2):322-332.
  6. Suematsu S, et al. Generation of a synthetic lymphoid tissue-like organoid in mice. (2004) Nat Biotechnol. 22(12):1539-1545.
  7. Yasui T, et al. Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light. (2004) Appl Opt. 43(14):2861-2867.
  8. Yamanouchi K, et al. Bone formation by transplanted human osteoblasts cultured within collagen sponge with dexamethasone in vitro. (2001) J Bone Miner Res. 16(5):857-867.
  9. Fujimoto E, et al. Beneficial effect of basic fibroblast growth factor on the repair of full-thickness defects in rabbit articular cartilage. (1999) Arch Orthop Trauma Surg. 119(3-4):139-145.
  10. Matsumoto A, et al. Induction of enamel matrix protein expression in an ameloblast cell line co-cultured with a mesenchymal cell line in vitro. (2011) In Vitro Cell Dev Biol Anim. 47(1):39-44.
  11. Nakamura M. et al. Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence. (2009) Bioconjug Chem. 20(9):1759-1764.
  12. Orisaka M. et al. Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle. (2006) Mol Reprod Dev. 73(6):737-744.
  13. Suzuki T. et al. Puromycin aminonucleoside induces apoptosis and increases HNE in cultured glomerular epithelial cells. (2001) Free Radic Biol Med. 31(5):615-623.
  14. Ishikawa H. et al. Psoriatic fibroblasts enhance cornified envelope formation in normal keratinocytes in vitro. (1997) Arch Dermatol Res, 289(9):551-553.
  15. Miyauchi Y. et al. Feasibility of in vitro culturing of lesional psoriatic keratinocytes in medium containing high calcium concentrations. (1995) Arch Dermatol Res. 287(8):731-734.
  16. Hatou S, et al. Functional Corneal Endothelium Derived from Corneal Stroma Stem Cells of Neural Crest Origin by Retinoic Acid and Wnt/β-Catenin Signaling. (2013) Stem Cells Dev. 22(5):828-839.
  17. Kimoto M, et al. Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic Acid 2-phosphate. (2012) Invest Ophthalmol Vis Sci. 53(12):7583-7589.
  18. Kaji H. et al. Electrically induced contraction of C2C12 myotubes cultured on a porous membrane-based substrate with muscle tissue-like stiffness. (2010) Biomaterials. 31(27):6981-6986.
  19. Hida N. et al. Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells. (2008) Stem Cells. 26(7):1695-1704.
  20. Kinebuchi Y. et al. Direct induction of layered tissues from mouse embryonic stem cells: potential for differentiation into urinary tract tissue. (2008) Cell Tissue Res. 331(3):605-615.
  21. Oyasu M. et al. Immunogold electron microscopic demonstration of distinct submembranous localization of the activated gammaPKC depending on the stimulation. (2008) J Histochem Cytochem. 56(3):253-265.
  22. Miyashita H. et al. Characterization of hair follicles induced in implanted, cultured rat keratinocyte sheets. (2004) Exp Dermatol. 13(8):491-498.
  23. Youdim KA. et al. Interaction between flavonoids and the blood-brainbarrier in vitro studies. (2003) J Neurochem. 85(1):180-192.
  24. Agu RU. et al. In-vitro nasal drug delivery studies: comparison of derivatised, fibrillar and polymerised collagen matrix-based human nasal primary culture 2 systems for nasal drug delivery studies. (2001) J Pharm Pharmacol. 53(11):1447-1456.
  25. Selvaratnam L. et al. Permeability barrier properties of oral keratinocyte cultures: a model of intact human oral mucosa. (2001) Oral Dis. 7(4):252-258.
  26. Kahn CR. et al. Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. (1993) Invest Ophthalmol Vis Sci. 34(12):3429-3441.

Collagen microspheres (15mL)

  1. Seto I, et al. Mandibular reconstruction using a combination graft of rhBMP-2 with bone marrow cells expanded in vitro. (2006) Plast Reconstr Surg. 117(3):902-908.
  2. Kanematsu A, et al. Type I collagen can function as a reservoir of basic fibroblast growth factor. (2004) J Control Release. 99(2):281-292.
  3. Konya E, et al. The role of osteopontin on calcium oxalate crystal formation. (2003) Eur Urol. 43(5):564-571.
  4. Overstreet M, et al. Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins. (2003) In Vitro Cell Dev Biol Anim. 39(5-6):228-234.
  5. Frondoza C, et al. Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture. (1996) Biomaterials. 17(9):879-888.

Specification Sheets


KKN-CSH-10Atelocollagen Honeycomb sponge (sterile)1537181036KKK-CSH-10.pdf
KKN-CSH-96Atelocollagen Honeycomb Disc 96 (sterile)
KKN-ACB-05SAtelocollagen coated β-TCP scaffold (sterile) Disc size : Φ3×1 mm1536831234KKK-ACB-05S.pdf
KKN-CSM-25Atelocollagen sponge, MGHTY 25 pcs (sterile)1536831264KKK-CSM-25.pdf
KKN-CSM-50Atelocollagen sponge, MGHTY 50 pcs (sterile)1536831286KKK-CSM-50.pdf
KKN-MEN-01Atelocollagen permeable membrane for 50 mm culture dish (sterile)
KKN-CM-6Atelocollagen membrane for 6-well culture plate (sterile)1536831348KKN-CM-6.pdf
KKN-CM-24Atelocollagen membrane for 24-well culture plate (sterile)1536831398KKN-CM-24.pdf
KKN-MIC-00Collagen microspheres (sterile) Size: 100-400 μm, About 3,000,000 particles, Total surface area: about 3,800 cm2/15 ml1536831321KKK-MIC-00.pdf

試劑 Solutions

KKN-IPC-30Atelocollagen, Bovine dermis, 3mg/mL (sterile)
KKN-IPC-50Atelocollagen, Bovine dermis, 5mg/mL (sterile)
KKN-IAC-30Native collagen, Bovine dermis, 3mg/mL (sterile)
KKN-IAC-50Native collagen, Bovine dermis, 5mg/mL (sterile)
KKN-MEN-02Atelocollagen, Eagle’s MEM (sterile)
KKN-DME-02Atelocollagen, DMEM Low Glucose (sterile)
KKN-DME-02HAtelocollagen, DMEM High Glucose (sterile)
KKN-RPM-02Atelocollagen RPMI 1640 (sterile)
KKN-CL-22Type II collagen (sterile) from bovine cartilage


KKN-CLS-01Atelocollagen sponge
KKN-CLF-01Atelocollagen membrane
KKN-CLP-01Atelocollagen powder