Ampli1™ WGA Kit 單顆細胞全基因組擴增套組

Ampli1™ WGA Kit 單細胞全基因組擴增 SCOMP (Single Cell Comparative Genomic Amplification) 的研究

• Mangano et al, Blood Cancer Journal 2019, "Precise detection of genomic imbalances at single-cell resolution reveals intra-patient heterogeneity in Hodgkin's lymphoma"
• Pailler et al, Clinical Cancer Research 2019, "Acquired Resistance Mutations to ALK-Inhibitors Identified by Single Circulating Tumor Cell Sequencing in ALK-Rearranged Non-Small-Cell Lung Cancer"
• Scharpenseel et al, Scientific Report 2019, "EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients"
• Faugeroux et al, European Urology Oncology 2019, "An Accessible and Unique Insight into Metastasis Mutational Content Through Whole-exome Sequencing of Circulating Tumor Cells in Metastatic Prostate Cancer"
• Rihawi et al, Translational Oncology 2019, "MYC Amplification as a Potential Mechanism of Primary Resistance to Crizotinib in ALK-Rearranged Non-Small Cell Lung Cancer: A Brief Report"
• Weidele et al, International Journal of Cancer 2018, "Microfluidic enrichment, isolation and characterization of disseminated melanoma cells from lymph node samples"
• Ferrarini A et al, PLOS ONE 2018, “A streamlined workflow for single-cells genome-wide copy-number profiling by low-pass sequencing of LM-PCR whole-genome amplification products”
• Werner-Klein et al, Nature Communications 2018, "Genetic alterations driving metastatic colony formation are acquired outside of the primary tumour in melanoma"
• Deleye et al , Scientific Reports 2017, “Performance of four modern whole genome amplification methods for copy number variant detection in single cells”
• Deleye et al, Scientific Reports 2018, "Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells"
• Paoletti et al, Cancer Research 2017, "Comprehensive mutation and copy number profiling in archived circulating breast cancer tumor cells documents heterogeneous resistance mechanisms"
• Pleatsen at al, Scientific Reports 2017, "STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods"
• Hoffmann et al, International Journal of Cancer 2017, “Diagnostic pathology of early systemic cancer: ERBB2 gene amplification in single disseminated cancer cells determines patient survival in operable esophageal cancer”
• Mesquita et al, Molecular Oncology 2017, “Molecular analysis of single circulating tumour cells following long-term storage of clinical samples”
• Mohme et al, Scientific Reports 2017, “Circulating Tumour Cell Release after Cement Augmentation of Vertebral Metastases”
• Kondo et al, BMC 2017, “KRAS mutation analysis of single circulating tumor cells from patients with metastatic colorectal cancer”
• Palmirotta et al, Cancer Genomics and Proteomics 2017, “Next-generation Sequencing (NGS) Analysis on Single Circulating Tumor Cells (CTCs) with No Need of Whole-genome Amplification (WGA)”
• Bingham C et al, Breast Cancer Res Treat 2017, “Mutational studies on single circulating tumor clles isolated form the blood of inflammatory breast cancer patients”
• Borgstrom E et al, PLOS One 2017, “Comparison of whole genome amplification techniques for human single cell exome sequencing”
• Chen S et al, Nature Scientific Reports 2017, “Catch and Release: rare cell analysis from a functionalised medical wire”
• Hosseini et al, Nature 2016, “Early dissemination seeds metastasis in breast cancer”
• Breman et al, Prenatal Diagnosis 2016, “Evidence for feasibility of fetal trophoblastic cell-based noninvasive prenatal testing”
• De Laere B et al, JTS 2016, “Patients with metastatic hormone receptor-positive breast cancer express PIK3CA oncogene mutational heterogeneity in circulating tumor cells”
• Carter L et al, Nature Medicine 2016, “Molecular analysis of circulating tumor cells identifies distinct copy-number profiles in patients with chemosensitive and chemorefractory small-cell lung cancer”
• Zhaomei Mu et al, IJMC 2016, “Detection and Characterization of Circulating Tumor Associated Cells in Metastatic Breast Cancer"
• Shaw J et al, Clinical Cancer Research 2016, “Mutation analysis of cell-free DNA and single circulating tumor cells in metastatic breast cancer patients with high CTC counts”
• Babayan et al, Oncotarget 2016, “Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells”
• Normand E et al, Prenatal Diagnosis 2016, “Comparison of three whole genome amplification methods for detection of genomic aberrations in single cells”
• Millner LM et al, Cancer Research Frontiers 2016, “Comprehensive isolation, identification, and nucleic acid analysis of single breast cancer cells: CTC-isoTECH”
• Neumann MHT et al, Biotechnology Progress 2016, “Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearch® system and the CellCelector™”
• De Luca F et al, Oncotarget 2016, “Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer”
• Bulfoni M et al, Breast Cancer Research 2016, “In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis”
• Rothwell G et al, Molecular Oncology 2015, “Genetic profiling of tumours using both circulating free DNA and circulating tumour cells isolated from the same preserved whole blood sample”
• Vishnoi M et al, Nature Scientific Reports 2015, “The isolation and characterization of CTC subsets related to breast cancer dormancy”
• Salvianti et al, Biomolecular Detection and Quantification 2015, "Feasibility of a workflow for the molecular characterization of singlecells by next generation sequencing”
• Hou Y ey al, Gigascience 2015, “Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing”
• Campton ED et al, BMC Cancer 2015, “High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining”
• Pestrin M et al, Molecular Oncology 2014, “Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients”
• Polzer B et al, EMBO Molecular Medicine 2014, “Molecular profiling of single circulating tumor cells with diagnostic intention”
• Neves R.P.L et al, Clinical Chemistry 2014, ”Genomic High-Resolution Profiling of Single vCKpos/CD45neg Flow-Sorting Purified Circulating Tumor Cells from Patients with Metastatic Breast Cancer”
• Fernandez S.V. et al, Breast Cancer Research 2014 , “TP53 mutations detected in circulating tumor cells present in the blood of metastatic triple negative breast cancer patients”
• Carpenter E, et al, Frontiers in Oncology 2014, “Dielectrophoretic capture and genetic analisys of single neuroblastoma tumor cells”
• Binder V. et al, Human Mutation 2014, “A new workflow for whole genome sequencing of single human cells”
• Hodgkinson C.L.et al, Nature Medicine 2014, “Tumorigenicity and genetic profiling of Circulating Tumor Cells in Small Cell Lung Cancer”
• Watanabe M et al, Journal of Translational Medicine 2014, “A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of circulating tumor cells”
• Czyz ZT, et al. PLOS One 2014, “Reliable single cell array CGH for clinical samples”
• Peeters DJE et al, British Journal of Cancer 2013, “Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting”
• Fabbri F, et al. Cancer Letters 2013, “Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs”
• Arneson N, et al. ISRN Oncology 2011 “Comparison of whole genome amplification methods for analysis of DNA extracted from microdissected early breast lesions in formalin-fixed paraffin-embedded tissue”
• Lee YS et al, Taiwanese Journal of Obstetrics & Gynecology 2008, “Comparison of whole genome amplification methods for further quantitative analysis with microarray-based comparative genome hybridization”
• Stoecklein NH, et al. Am J Pathol 2002 “SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples”
• Klein CA, et al. Proc Natl Acad Sci USA 1999 “Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells”

Brochure

• Ampli1 WGA Kit_Brochure.pdf
• Silicon_Biosystems_Ampli1_Brochure_2015.pdf

Technical Bulletin

• SILICON_TECHNICAL_BULLETIN_WGA_ftoA4_20180330_ver_WEB.pdf
• Ampli1 Technical Bulletin PDF.pdf

FAQs

• Ampli1_FAQs.docx